GeneSpark™ Human HSPA6 Promoter Primers (VS-0725-FY83)
CAT#: VS-0725-FY83
The Human HSPA6 Promoter Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human HSPA6 (Heat Shock Protein Family A Member 6) gene, also known as HSP70B'. HSPA6 is a heat-inducible molecular chaperone involved in cellular stress responses. Its promoter is highly responsive to heat shock and other stressors, making it an excellent target for studying dynamic gene regulation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In essence, primers are the navigational tools in ChIP assays, transforming a complex biological question into a quantifiable answer. They enable the specific amplification and detection of DNA sequences enriched by immunoprecipitation, providing invaluable insights into how proteins interact with DNA to regulate gene expression, maintain genome integrity, and orchestrate cellular processes. Their precision and reliability are what empower researchers to dissect the intricate mechanisms of gene regulation and unravel the fundamental blueprints of life.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Heat Shock Protein Family A (Hsp70) Member 6
- Introduction
- HSPA6 (Heat Shock Protein Family A (Hsp70) Member 6) is a Protein Coding gene. Among its related pathways are Innate Immune System and MAPK signaling pathway. Gene Ontology (GO) annotations related to this gene include enzyme binding and heat shock protein binding. An important paralog of this gene is HSPA1B.
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 6; Heat Shock 70kDa Protein 6 (HSP70B); Heat Shock 70kD Protein 6 (HSP70B); Heat Shock 70 KDa Protein B; HSP70B; Heat Shock 70 KDa Protein 6
- Gene ID
- 3310
- UniProt ID
- P17066
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