GeneSpark™ Human KLK2 Intron 1 Primers (VS-0725-FY54)
CAT#: VS-0725-FY54
The Human KLK2 Intron 1 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target intron 1 of the human KLK2 (Kallikrein-related peptidase 2) gene. KLK2 is a serine protease, also known as human glandular kallikrein 2, primarily expressed in the prostate. Its expression is often androgen-regulated, making it relevant for studying hormone-responsive gene regulation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers in ChIP assays are highly specific probes. Their main function is to detect and quantify particular DNA sequences that have been isolated via immunoprecipitation, ultimately revealing crucial protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Kallikrein Related Peptidase 2
- Introduction
- This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants.
- Alternative Names
- Kallikrein Related Peptidase 2; Kallikrein 2, Prostatic; Glandular Kallikrein-1; Tissue Kallikrein-2; HGK-1; Glandular Kallikrein 2
- Gene ID
- 3817
- UniProt ID
- P20151
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