GeneSpark™ Human MS4A7 Promoter Primers (VS-0725-FY64)
CAT#: VS-0725-FY64
The Human MS4A7 Promoter Primers offer a precise solution for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product includes optimized forward and reverse PCR primers specific to the promoter region of the human MS4A7 (Membrane Spanning 4-Domains A7) gene. MS4A7 is a member of the MS4A gene family, primarily expressed in myeloid cells, and has been implicated in immune responses and inflammation. These primers are optimized for SYBR® Green quantitative real-time PCR and are thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They facilitate the investigation of epigenetic regulation in immune cell types.
Specifications
- Application
- ChIP
- Application Notes
- In ChIP assays, primers act as highly specific probes designed to identify and quantify particular DNA sequences that have been isolated through immunoprecipitation, thereby uncovering vital protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Membrane Spanning 4-Domains A7
- Introduction
- This gene encodes a member of the membrane-spanning 4A gene family, members of which are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns in hematopoietic cells and nonlymphoid tissues. This family member is associated with mature cellular function in the monocytic lineage, and it may be a component of a receptor complex involved in signal transduction. This gene is localized to 11q12, in a cluster of other family members. At least four alternatively spliced transcript variants encoding two distinct isoforms have been observed.
- Alternative Names
- Membrane Spanning 4-Domains A7; Four-Span Transmembrane Protein 2; CD20 Antigen-Like 4; 4SPAN2; CD20L4; CFFM4; High Affinity Immunoglobulin Epsilon Receptor Beta Subunit
- Gene ID
- 58475
- UniProt ID
- Q9GZW8
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