GeneSpark™ Human MX1 Promoter Primers (VS-0725-FY56)
CAT#: VS-0725-FY56
The Human MX1 Promoter Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human MX1 (MX Dynamin Like GTPase 1) gene. MX1 is a key interferon-stimulated gene, playing a crucial antiviral role. Its robust induction upon viral infection makes its promoter an excellent target for studying rapid and dynamic gene regulation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- The primary role of primers in chromatin immunoprecipitation (ChIP) assays is to act as accurate detectors. They enable the identification and measurement of specific DNA sequences that have been concentrated through immunoprecipitation, thereby illuminating protein-DNA binding events.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- MX Dynamin Like GTPase 1
- Introduction
- This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.
- Alternative Names
- MX Dynamin Like GTPase 1; Interferon-Regulated Resistance GTP-Binding Protein MxA; Interferon-Inducible Protein P78; Interferon-Induced Protein P78; Myxoma Resistance Protein 1; IFI-78K; Myxovirus (Influenza) Resistance 1, Homolog Of Murine (Interferon-Inducible Protein P78); Myxovirus (Influenza Virus) Resistance 1, Interferon-Inducible Protein P78 (Mouse)
- Gene ID
- 4599
- UniProt ID
- P20591
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