GeneSpark™ Human Negative Control Primer Set 3 (VS-0725-FY106)
CAT#: VS-0725-FY106
The Human Negative Control Primer Set 3 offers a third, independent option for establishing a reliable negative control in human ChIP assays. Comprising a validated mixture of forward and reverse primers, this set targets a distinct genomic locus that should not be enriched in typical ChIP experiments. Its inclusion helps confirm the absence of non-specific amplification and background noise, providing essential data for the accurate interpretation and validation of specific protein-DNA interactions.
Specifications
- Application
- ChIP
- Application Notes
- Designed specifically for human samples, Human Control qPCR Primer Sets act as essential positive or negative controls in ChIP experiments. Each set contains validated forward and reverse primers, ensuring reliable performance in both qPCR and endpoint PCR applications for ChIP samples derived from multiple human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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