GeneSpark™ Human NPM1 Intron 1 Primers (VS-0725-FY93)
CAT#: VS-0725-FY93
The Human NPM1 Intron 1 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting intron 1 of the human NPM1 (Nucleophosmin 1) gene. NPM1 is a multifunctional nucleolar protein involved in ribosome biogenesis, chromatin remodeling, and genome stability, often mutated in acute myeloid leukemia. Investigating intronic regions can provide insights into chromatin state or RNA polymerase progression within the gene body. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- At the heart of Chromatin Immunoprecipitation (ChIP) assays, primers stand as sophisticated detectors. They empower researchers to accurately pinpoint and quantify the specific DNA sequences pulled down through immunoprecipitation, thereby illuminating the direct interplay between proteins and DNA.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Nucleophosmin 1
- Introduction
- The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
- Alternative Names
- Nucleophosmin 1; Nucleophosmin (Nucleolar Phosphoprotein B23, Numatrin); Nucleophosmin/Nucleoplasmin Family, Member 1; Nucleolar Phosphoprotein B23; Nucleolar Protein NO38; Nucleophosmin
- Gene ID
- 4869
- UniProt ID
- P06748
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