GeneSpark™ Human NR4A3 Promoter Primers (VS-0725-FY92)
CAT#: VS-0725-FY92
The Human NR4A3 Promoter Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human NR4A3 (Nuclear Receptor Subfamily 4 Group A Member 3) gene. NR4A3 acts as a transcription factor, rapidly induced by various stimuli and involved in apoptosis, inflammation, and cell proliferation. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- At the heart of Chromatin Immunoprecipitation (ChIP) assays, primers stand as sophisticated detectors. They empower researchers to accurately pinpoint and quantify the specific DNA sequences pulled down through immunoprecipitation, thereby illuminating the direct interplay between proteins and DNA.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Nuclear Receptor Subfamily 4 Group A Member 3
- Introduction
- This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Multiple transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- Nuclear Receptor Subfamily 4 Group A Member 3; Mitogen-Induced Nuclear Orphan Receptor; Neuron-Derived Orphan Receptor 1; Nuclear Hormone Receptor NOR-1; MINOR; CSMF; NOR1
- Gene ID
- 8013
- UniProt ID
- Q92570
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