GeneSpark™ Human PabPC1 Exon 1 Primers (VS-0725-FY76)
CAT#: VS-0725-FY76
The Human PabPC1 Exon 1 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting exon 1 of the human PabPC1 (Poly(A)-Binding Protein Cytoplasmic 1) gene. PABPC1 is a crucial RNA-binding protein involved in mRNA stability, translation initiation, and poly(A) tail metabolism. Targeting an exonic region can offer insights into the chromatin state of actively transcribed areas or specific protein binding within the gene body. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In the intricate world of molecular biology, Chromatin Immunoprecipitation (ChIP) assays stand out as a powerful technique for unraveling the complex dance between proteins and DNA. At the heart of ChIP's success lies the primer, a small but mighty oligonucleotide that plays a pivotal role in the identification and quantification of specific DNA sequences. Understanding the function of primers is key to appreciating the depth of information ChIP can provide about protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Poly(A) Binding Protein Cytoplasmic 1
- Introduction
- This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
- Alternative Names
- Poly(A) Binding Protein Cytoplasmic 1; PABPC2; PABP1; PAB1; Poly(A)-Binding Protein, Cytoplasmic 2; Poly(A) Binding Protein, Cytoplasmic 2
- Gene ID
- 26986
- UniProt ID
- P11940
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