GeneSpark™ Human Positive Control Primer Set ACTB-1 (VS-0725-FY107)
CAT#: VS-0725-FY107
The Human Positive Control Primer Set ACTB-1 is an essential positive control for human chromatin immunoprecipitation (ChIP) assays. This set includes a precisely validated blend of forward and reverse primers targeting a well-characterized, consistently bound region within the ACTB (beta-actin) gene. It guarantees robust and consistent amplification in both qPCR and endpoint PCR, making it ideal for verifying the overall success and efficiency of your ChIP experiment and confirming the proper enrichment of immunoprecipitated DNA.
Specifications
- Application
- ChIP
- Application Notes
- Designed specifically for human samples, Human Control qPCR Primer Sets act as essential positive or negative controls in ChIP experiments. Each set contains validated forward and reverse primers, ensuring reliable performance in both qPCR and endpoint PCR applications for ChIP samples derived from multiple human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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