GeneSpark™ Human Positive Control Primer Set GEMIN4 (VS-0725-FY112)
CAT#: VS-0725-FY112
The Human Positive Control Primer Set GEMIN4 is specifically formulated as a positive control for chromatin immunoprecipitation (ChIP) in human samples. This primer set features a validated mixture of forward and reverse primers that target a specific, highly bound region of the GEMIN4 gene. It facilitates consistent and accurate amplification in both qPCR and endpoint PCR, crucial for confirming the efficiency of your ChIP protocol and validating the quality of your immunoprecipitated DNA.
Specifications
- Application
- ChIP
- Application Notes
- Designed specifically for human samples, Human Control qPCR Primer Sets act as essential positive or negative controls in ChIP experiments. Each set contains validated forward and reverse primers, ensuring reliable performance in both qPCR and endpoint PCR applications for ChIP samples derived from multiple human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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