GeneSpark™ Human Positive Control Primer Set ZC3H13 (VS-0725-FY114)
CAT#: VS-0725-FY114
Designed as an effective positive control for human chromatin immunoprecipitation (ChIP) experiments, the Human Positive Control Primer Set ZC3H13 offers dependable performance. This primer set includes a validated mixture of forward and reverse primers that specifically target a known enriched region of the ZC3H13 gene. It ensures precise amplification in both qPCR and endpoint PCR, allowing researchers to reliably verify the success of their immunoprecipitation and the presence of enriched DNA.
Specifications
- Application
- ChIP
- Application Notes
- When performing chromatin immunoprecipitation (ChIP) with human samples, Human Control qPCR Primer Sets serve as critical positive or negative controls. These sets feature validated mixtures of forward and reverse primers, proven effective for qPCR and endpoint PCR analysis of ChIP samples from diverse human cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Human
- Storage
- Store at -20°C.
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