GeneSpark™ Human PRF1 Promoter Primers (VS-0725-FY63)
CAT#: VS-0725-FY63
The Human PRF1 Promoter Primers provide a specific tool for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human PRF1 (Perforin 1) gene. Perforin is a key cytotoxic protein released by natural killer cells and cytotoxic T lymphocytes, crucial for immune defense against viral infections and tumors. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are rigorously validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are valuable for studying immune gene regulation.
Specifications
- Application
- ChIP
- Application Notes
- In ChIP assays, primers act as highly specific probes designed to identify and quantify particular DNA sequences that have been isolated through immunoprecipitation, thereby uncovering vital protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- perforin 1
- Introduction
- This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate.
- Alternative Names
- perforin 1; PRF1; human Perforin
- Gene ID
- 5551
- UniProt ID
- P14222
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY63. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Related Products
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA, FC, Inhib, FuncS
-3.jpg)
Application: IB, FC, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
