GeneSpark™ Human SLC19A2 Promoter Primers (VS-0725-FY77)
CAT#: VS-0725-FY77
The Human SLC19A2 Promoter Primers are designed for amplifying DNA isolated through chromatin immunoprecipitation (ChIP). This product includes optimized forward and reverse PCR primers specific to the promoter region of the human SLC19A2 (Solute Carrier Family 19 Member 2) gene, encoding the thiamine transporter THTR1. SLC19A2 is essential for cellular thiamine uptake, and mutations are linked to Rogers syndrome. Studying its promoter is crucial for understanding its transcriptional control. These primers are optimized for SYBR® Green quantitative real-time PCR and are thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In the intricate world of molecular biology, Chromatin Immunoprecipitation (ChIP) assays stand out as a powerful technique for unraveling the complex dance between proteins and DNA. At the heart of ChIP's success lies the primer, a small but mighty oligonucleotide that plays a pivotal role in the identification and quantification of specific DNA sequences. Understanding the function of primers is key to appreciating the depth of information ChIP can provide about protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Solute Carrier Family 19 Member 2
- Introduction
- This gene encodes the thiamin transporter protein. Mutations in this gene cause thiamin-responsive megaloblastic anemia syndrome (TRMA), which is an autosomal recessive disorder characterized by diabetes mellitus, megaloblastic anemia and sensorineural deafness. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- Solute Carrier Family 19 Member 2; Solute Carrier Family 19 (Thiamine Transporter), Member 2; ThTr-1; THTR1; TRMA; THT1; TC1
- Gene ID
- 10560
- UniProt ID
- O60779
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