GeneSpark™ Human TAP1 Promoter Primers (VS-0725-FY82)
CAT#: VS-0725-FY82
The Human TAP1 Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human TAP1 (Transporter Associated With Antigen Processing 1) gene. TAP1 is a key component of the MHC class I antigen processing pathway, essential for presenting viral and tumor antigens to T cells. Its expression is often regulated by interferons. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In essence, primers are the navigational tools in ChIP assays, transforming a complex biological question into a quantifiable answer. They enable the specific amplification and detection of DNA sequences enriched by immunoprecipitation, providing invaluable insights into how proteins interact with DNA to regulate gene expression, maintain genome integrity, and orchestrate cellular processes. Their precision and reliability are what empower researchers to dissect the intricate mechanisms of gene regulation and unravel the fundamental blueprints of life.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Transporter 1, ATP Binding Cassette Subfamily B Member
- Introduction
- The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- Transporter 1, ATP Binding Cassette Subfamily B Member; Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP); Peptide Transporter Involved In Antigen Processing 1; ATP-Binding Cassette Sub-Family B Member 2; Really Interesting New Gene 4 Protein; Peptide Transporter PSF1; Peptide Transporter TAP1; Peptide Supply Factor 1; ABCB2; PSF-1; RING4; APT1; PSF1
- Gene ID
- 6890
- UniProt ID
- Q03518
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