GeneSpark™ Human TRIO Exon 57 Primers (VS-0725-FY52)
CAT#: VS-0725-FY52
The Human TRIO Exon 57 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target exon 57 of the human TRIO (Triple GEF and Rho GDP/GTP Exchange Factor) gene. TRIO is a large protein involved in actin cytoskeleton remodeling and cell migration, with multiple isoforms. Targeting a specific exon can be useful for assessing gene body chromatin marks or RNA polymerase presence. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In ChIP assays, primers are essential for accurately identifying and quantifying specific DNA sequences. These sequences are enriched during immunoprecipitation, allowing researchers to effectively map out protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Trio Rho Guanine Nucleotide Exchange Factor
- Introduction
- This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants.
- Alternative Names
- Trio Rho Guanine Nucleotide Exchange Factor; Triple Functional Domain (PTPRF Interacting); PTPRF-Interacting Protein; EC 2.7.11.1; Triple Functional Domain Protein; ARHGEF23; MEBAS; MRD44; Tgat
- Gene ID
- 7204
- UniProt ID
- O75962
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