GeneSpark™ Mouse AFM Intron 2 Primers (VS-0725-FY51)
CAT#: VS-0725-FY51
The Mouse AFM Intron 2 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting intron 2 of the mouse AFM (Afamin) gene. Afamin is a glycoprotein belonging to the albumin gene family, primarily expressed in the liver and involved in vitamin E transport. Investigating intronic regions can provide insights into chromatin state or gene body transcription. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In ChIP assays, primers are essential for accurately identifying and quantifying specific DNA sequences. These sequences are enriched during immunoprecipitation, allowing researchers to effectively map out protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Afamin
- Introduction
- This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
- Alternative Names
- Afamin; Alpha-Albumin; Alpha-Alb; ALB2; ALBA; ALF
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