GeneSpark™ Mouse Exo1 Exon 12 Primers (VS-0725-FY90)
CAT#: VS-0725-FY90
The Mouse Exo1 Exon 12 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target exon 12 of the mouse Exo1 (Exonuclease 1) gene. Exo1 is a 5'-3' exonuclease involved in DNA mismatch repair, homologous recombination, and DNA damage signaling. Targeting a specific exon can be useful for assessing gene body chromatin marks or the presence of RNA polymerase. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers serve a fundamental role in ChIP assays by acting as highly accurate probes. They are essential for precisely identifying and measuring the specific DNA sequences that have been concentrated via immunoprecipitation, ultimately unveiling the intricate details of protein-DNA binding events.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Exonuclease 1
- Introduction
- This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.
- Alternative Names
- Exonuclease 1; Rad2 Nuclease Family Member, Homolog Of S. Cerevisiae Exonuclease 1; HExoI; HEX1; Exonuclease I; EC 3.1.-.-; HExo1; EXOI
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