GeneSpark™ Mouse HoxA1 Promoter Primers (VS-0725-FY74)
CAT#: VS-0725-FY74
The Mouse HoxA1 Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the mouse HoxA1 gene. HoxA1 is a homeobox gene critical for embryonic development, particularly in hindbrain patterning and axial skeleton formation. Its precise transcriptional regulation is vital for normal development. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been rigorously tested with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are invaluable for studying the epigenetic regulation of developmental genes in mice.
Specifications
- Application
- ChIP
- Application Notes
- Primers play a pivotal role in chromatin immunoprecipitation (ChIP) assays. They serve as precise tools designed to detect and quantify specific DNA sequences that have been enriched through the immunoprecipitation process. This targeted detection is vital for uncovering and mapping protein-DNA interactions, offering crucial insights into gene regulation and chromatin structure. Essentially, primers enable researchers to pinpoint the exact locations where proteins bind to DNA.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Homeobox A1
- Introduction
- In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region.
- Alternative Names
- Homeobox A1; Homeobox Protein Hox-1F; Homeo Box A1; HOX1F; HOX A1 Homeodomain Protein; Homeobox Protein Hox-A1
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