GeneSpark™ Mouse Intracisternal A-Particle (IAP) LTR Primers (VS-0725-FY43)
CAT#: VS-0725-FY43
The Mouse Intracisternal A-Particle (IAP) LTR Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting the Long Terminal Repeat (LTR) regions of Mouse Intracisternal A-Particles (IAPs). IAPs are retrotransposons, and their LTRs are often used to study the epigenetic regulation of repetitive elements in the mouse genome, which are frequently heterochromatic. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- The primary role of primers in chromatin immunoprecipitation (ChIP) assays is to act as precise probes for identifying and quantifying particular DNA sequences that have been enriched through immunoprecipitation, thereby uncovering protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
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