GeneSpark™ Mouse MYT-1 Promoter Primers (VS-0725-FY61)
CAT#: VS-0725-FY61
The Mouse MYT-1 Promoter Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting the promoter region of the mouse MYT-1 (Myelin Transcription Factor 1) gene. MYT-1 is a transcription factor crucial for nervous system development, particularly in oligodendrocyte differentiation. Its precise transcriptional regulation is essential for proper neural cell fate. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- The primary function of primers in chromatin immunoprecipitation (ChIP) experiments is to serve as specific probes for detecting and quantifying particular DNA fragments enriched after immunoprecipitation, thereby revealing protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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