GeneSpark™ Mouse Negative Control Primer Set 4 (VS-0725-FY118)
CAT#: VS-0725-FY118
As a further option for negative control in mouse ChIP assays, the Mouse Negative Control Primer Set 4 offers dependable performance. This primer set contains a validated mixture of forward and reverse primers targeting a fourth distinct genomic area not expected to be bound by typical proteins. Its use is key to thoroughly assessing background levels and reinforcing the specificity of ChIP results, ensuring high confidence in the identification of genuine protein-DNA interactions.
Specifications
- Application
- ChIP
- Application Notes
- Mouse Control qPCR Primer Sets are specifically designed to act as either positive or negative controls in chromatin immunoprecipitation (ChIP) experiments when working with mouse samples. Each primer set is a combination of forward and reverse primers that have been validated for both quantitative polymerase chain reaction (qPCR) and endpoint PCR of ChIP samples derived from various mouse cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY118. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, Neut
-4.jpg)
Application: FC
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
Application: FC
Application: ELISA, Neut, FuncS
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
