GeneSpark™ Mouse Positive Control Primer Set Actb-2 (VS-0725-FY120)
CAT#: VS-0725-FY120
As another highly reliable positive control for mouse ChIP applications, the Mouse Positive Control Primer Set Actb-2 targets an alternative, distinct region of the mouse Actb (beta-actin) gene. This set features a meticulously validated mixture of forward and reverse primers, ensuring strong and consistent amplification in both quantitative and endpoint PCR analyses. It's an excellent tool for confirming the successful enrichment of target DNA during immunoprecipitation, providing a critical benchmark for experimental validation and quality control.
Specifications
- Application
- ChIP
- Application Notes
- Mouse Control qPCR Primer Sets are specifically designed to act as either positive or negative controls in chromatin immunoprecipitation (ChIP) experiments when working with mouse samples. Each primer set is a combination of forward and reverse primers that have been validated for both quantitative polymerase chain reaction (qPCR) and endpoint PCR of ChIP samples derived from various mouse cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
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