GeneSpark™ Mouse Positive Control Primer Set Pax-2 (VS-0725-FY125)
CAT#: VS-0725-FY125
The Mouse Positive Control Primer Set Pax-2 is specifically formulated as a robust positive control for chromatin immunoprecipitation (ChIP) in mouse samples. This primer set features a validated mixture of forward and reverse primers that target a specific, highly bound region of the mouse Pax-2 gene. It facilitates consistent and accurate amplification in both qPCR and endpoint PCR, crucial for confirming the efficiency of your ChIP protocol and validating the quality of your immunoprecipitated DNA.
Specifications
- Application
- ChIP
- Application Notes
- Mouse Control qPCR Primer Sets are specifically designed to act as either positive or negative controls in chromatin immunoprecipitation (ChIP) experiments when working with mouse samples. Each primer set is a combination of forward and reverse primers that have been validated for both quantitative polymerase chain reaction (qPCR) and endpoint PCR of ChIP samples derived from various mouse cell lines.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 96 rxns
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
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