GeneSpark™ Mouse TULP4 Promoter Primers (VS-0725-FY65)
CAT#: VS-0725-FY65
The Mouse TULP4 Promoter Primers are designed for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers specifically targeting the promoter region of the mouse TULP4 (Tubby Like Protein 4) gene. TULP4 is known to play a role in DNA damage response and mitosis, associating with centrosomes and microtubules. These primers are optimized for use in SYBR® Green quantitative real-time PCR and have been rigorously tested with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are valuable for studying the chromatin landscape and regulation of genes involved in genome stability.
Specifications
- Application
- ChIP
- Application Notes
- In chromatin immunoprecipitation (ChIP) assays, primers are crucial for accurately detecting and quantifying particular DNA sequences. These sequences are enriched during immunoprecipitation, enabling researchers to effectively map protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Tubby Like Protein 4
- Introduction
- TULP4 (Tubby Like Protein 4) is a Protein Coding gene. Diseases associated with TULP4 include Type 1 Diabetes Mellitus 5 and Cranioectodermal Dysplasia 1. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity. An important paralog of this gene is WDR35.
- Alternative Names
- Tubby Like Protein 4; Tubby Superfamily Protein; TUSP; Tubby Super-Family Protein; Tubby-Related Protein 4; Tubby-Like Protein 4; KIAA1397; TUBL4
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