GeneSpark™ Mouse XIST Intron 1 Primers (VS-0725-FY87)
CAT#: VS-0725-FY87
The Mouse XIST Intron 1 Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target intron 1 of the mouse XIST (X-Inactive Specific Transcript) gene. XIST is a long non-coding RNA essential for X-chromosome inactivation, where one of the two X chromosomes in female mammals is silenced. This region is typically associated with repressive chromatin marks. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In qPCR, the primers enable the exponential copying of the target DNA, allowing for its precise quantification. The amount of amplified DNA is directly proportional to the initial amount of target DNA present in the immunoprecipitated sample. This quantitative measure is incredibly valuable, as it not only tells us if a protein binds to a specific DNA region, but also how strongly or how frequently that binding occurs under different conditions.
For ChIP-seq, primers are used to generate libraries of DNA fragments that are then sequenced. This provides an even broader, genome-wide view of protein-DNA binding sites, and primers are essential for preparing these fragments for the sequencing process.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- X Inactive Specific Transcript (Non-Protein Coding)
- Introduction
- X inactivation is an early developmental process in mammalian females that transcriptionally silences one of the pair of X chromosomes, thus providing dosage equivalence between males and females. The process is regulated by several factors, including a region of chromosome X called the X inactivation center (XIC). The XIC comprises several non-coding and protein-coding genes, and this gene was the first non-coding gene identified within the XIC. This gene is expressed exclusively from the XIC of the inactive X chromosome, and is essential for the initiation and spread of X-inactivation. The transcript is a spliced RNA. Alternatively spliced transcript variants have been identified, but their full length sequences have not been determined. Mutations in the XIST promoter cause familial skewed X inactivation.
- Alternative Names
- X Inactive Specific Transcript (Non-Protein Coding) 2 3 5 Long Intergenic Non-Protein Coding RNA 1 2 3 X (Inactive)-Specific Transcript (Non-Protein Coding) 2 X (Inactive)-Specific Transcript 2 NCRNA00001 3 LINC00001 3
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY87. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Related Products
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, IF, FuncS
Application: ELISA, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IF, Block, FuncS
Application: ELISA, WB, Microarray, Block
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
