Human Anti-IL33 (clone W25scFv88) scFv-Fc Chimera (CAT#: VS-0125-FY37)
This is a developed recombinant vectors for the eukaryotic expression of single-chain variable fragments (scFv-Fcs) targeting IL-33. The produced scFv-Fcs demonstrate strong binding affinity for human IL-33, making them promising candidates for the development of innovative therapies aimed at treating allergic and autoimmune diseases. This advancement highlights the potential of scFv-Fcs in addressing unmet medical needs in this therapeutic area.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1907_B1_4_selected.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1907_B1_4_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24426/53688_A_7_3_selected.jpg
(Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24426/53688_A_7_3_selected.jpg
Figure 3 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1936_B1_1_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1936_B1_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1953_C1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1953_C1_1_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_8_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_3.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_3.jpg
Figure 7 Endometrium
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_4_3.jpg
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_4_3.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_8.jpg
Figure 9 RNA cell line category: Group enriched (hTERT-RPE1, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137033-IL33
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137033-IL33
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- W25scFv88
- Applications
- Immunoassay
- Related Disease
- Autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The correctly folded scFv-Fcs with strong binding affinity for human IL-33 stands out as a promising candidate for the development of a novel therapy targeting allergic and autoimmune conditions.
Target
- Alternative Names
- Interleukin 33
- Gene ID
- 90865
- UniProt ID
- O95760
- Sequence Similarities
- Belongs to the IL-1 family. Highly divergent.
- Cellular Localization
- Chromosome, Cytoplasm, Cytoplasmic vesicle, Nucleus, Secreted
- Post Translation Modifications
- The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein.
- Protein Refseq
- NP_001186569.1
- Function
- Acts as an enhancer of polarization of alternatively activated macrophages.
Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-769-sdAb | Recombinant Anti-Mouse Il33 VHH Single Domain Antibody | ICC, ChiP, FC, FA | Llama VHH |
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