Human Anti-RHOA/B (clone F7) scFv-Fc Chimera (CAT#: VS-0325-FY166)

• Immunogen
• GTP-locked mutant RhoAL63.

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Specificity
• Human RHOA/B

• Species Reactivity
• Human

• Clone
• F7

• Applications
• ELISA, Western Blot

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Alternative Names
• RHOA, RHOB; Human RhoA and RhoB proteins

• Long Name
• Human RhoA and RhoB proteins

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rho family.

• Cellular Localization
• Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane; Nucleus

• Post Translation Modifications
• (Microbial infection) Substrate for botulinum ADP-ribosyltransferase.
  (Microbial infection) AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively.
  AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells.

• Protein Refseq
• NP_001300870.1; NP_001300872.1; NP_001300873.1; NP_001300874.1; NP_001300875.1

• Function
• Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:8910519, PubMed:9121475, PubMed:31570889).
  Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed:16236794, PubMed:12900402).
  Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion.

Isotype Control

• MOB-200CQ
• Human IgG Fc Isotype Control Antibody

Secondary Antibody

• 2ND-029CQ
• Goat Anti-Human IgG Fc Antibody