Recombinant Mouse Anti-NFE2L1 Antibody (VS4-CJ52) (CAT#: VS4-CJ52)

• Immunogen
• Purified recombinant NRF1 protein fragments expressed in E. coli

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human NFE2L1

• Species Reactivity
• Human

• Applications
• ELISA, WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.1% BSA, PBS, pH 7.4.

• Preservative
• 0.2% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000).

• Alternative Names
• NRF1; NRF-1; TCF11; LCR-F1; alpha pal; alpha palindromic binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; nuclear factor (erythroid derived 2)-like 1; nuclear respiratory factor 1; transcription factor 11

• Gene ID
• 4779

• UniProt ID
• Q14494

• Long Name
• NFE2 like bZIP transcription factor 1

• Sequence Similarities
• Belongs to the bZIP family. CNC subfamily.

• Cellular Localization
• Endoplasmic reticulum; Membrane; Nucleus

• Post Translation Modifications
• Endoplasmic reticulum membrane sensor NFE2L1
  Cleaved at Leu-104 by the aspartyl protease DDI2 following retrotranslocation, releasing the protein from the endoplasmic reticulum membrane and forming the transcription factor NRF1 that translocates into the nucleus (PubMed:24448410, PubMed:27676297, PubMed:27676298, PubMed:27528193).
  Ubiquitination is prerequisite for cleavage by aspartyl protease DDI2 (PubMed:27676298).
  N-glycosylated in normal conditions, when it has a single-pass type II membrane protein topology, with the DNA-binding domain facing the endoplasmic reticulum lumen (PubMed:20932482, PubMed:24998528, PubMed:24448410, PubMed:27528193).
  Deglycosylated during retrotranslocation to the cytosolic side of the membrane, to have a single-pass type III membrane protein topology with the major part of the protein facing the cytosol (PubMed:20932482, PubMed:24998528, PubMed:24448410).
  Ubiquitinated by the SCF(FBXW7) complex and SYVN1/HRD1, leading to its degradation by the proteasome (PubMed:20932482).
  Ubiquitinated during retrotranslocation to the cytosolic side of the membrane: ubiquitination does not lead to degradation and is required for processing by the aspartyl protease DDI2 and subsequent release from the endoplasmic reticulum membrane (PubMed:24998528, PubMed:27676298).

• Protein Refseq
• NP_001317191.1; NP_003195.1

• Function
• Endoplasmic reticulum membrane sensor NFE2L1:
  Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor (PubMed:20932482, PubMed:24448410).
  Constitutes a precursor of the transcription factor NRF1 (By similarity).
  Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition (PubMed:20932482).
  In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1 (By similarity).
  Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36 (By similarity).
  Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits (PubMed:20932482).

Secondary Antibody

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS