KSHV vIL-6

Kaposi's sarcoma-as-sociated herpesvirus (KSHV), a human herpesvirus type 8 (hhv-8), is a causative agent of Kaposi's sacoma (KS), and is closely related to the occurrence of primary effusive lymphoma (PEL) and multi-center Castleman. Viral interleukin 6(vil-6) and human il-6 (hll-6), encoded by KSHV early open reading frame (ORF)K2 gene, have about 25% homology and contain multiple conserved amino acid sites associated with the function of hll-6, such as blocking cell apoptosis, inducing protein expression in acute phase and promoting the growth of hll-6 dependent cells. More importantly, vll-6 has been shown to be an important tumorigenic protein encoded by KSHV. It has been reported that mouse embryonic fibroblasts with a stable expression of vll-6 were inoculated under the skin of nude mice, which could induce tumor formation. Recent studies have also demonstrated that transactive protein (Tat) and negative regulatory factor (Nef) of hiv-1 can cooperate with KSHV vll-6 to promote cell proliferation, angiogenesis and tumor formation. In order to study the role of vll-6 in the tumorigenic process of KSHV, the anti-vll-6 polyclonal antibody is prepared by using artificial peptide as hema in this study, and it is initially applied to specifically detect KSHV vll-6 protein.