MTX

Methotrexate (MTX) is a clinically important antifolate drug, which competitively inhibits dihydrofolate reductase (DHFR), thereby impairing folate production. Folate is necessary for the de novo synthesis of thymidine, required for DNA synthesis. Folate is also required for purine base synthesis of DNA, RNA, thymidylates, and proteins. Thus, methotrexate impairs synthesis of thymidine and other metabolites crucial for cell proliferation.