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    Hi-Affi™ Rabbit Anti-ATP5B Recombinant Antibody (clone DS297AB) (CAT#: MOR-0297)

    Recombinant Rabbit anti-ATP5B monoclonal antibody for WB, IHC-P, ICC/IF, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1520/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human stomach
    Figure 2 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows strong positivity in granular pattern in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17527/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1528/4705_B_7_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1520/4419_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1528/4705_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1528/4705_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Round or early spermatids
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Sertoli cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1528/4705_A_5_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1528/4705_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110955-ATP5F1B

    Specifications

    • Immunogen
    • Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATPB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    • Host Species
    • Rabbit
    • Derivation
    • Phage display library screening
    • Type
    • Rabbit IgG
    • Species Reactivity
    • Mouse, Rat, Human
    • Clone
    • DS297AB
    • Applications
    • WB, IHC-P, IF, IP
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Format
    • Liquid
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Beta Polypeptide; EC 3.6.3.14; ATPSB; ATPMB; Mitochondrial ATP Synthetase, Beta Subunit; Mitochondrial ATP Synthase Beta Subunit; Epididymis Secretory Protein Li 271; HEL-S-271; EC 3.6.3

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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