Anti-B. anthracis toxin Recombinant Antibody (32E12)

CHO-derived antibodies specific for PA were preincubated with fixed amounts of PA and LF prior to their addition to cultures of RAW 264.7 cells. Cell viability was determined approximately 16 h later, and the degree to which the added antibody blocked toxicity was determined. The domain specificity (PA₂₀, PA₆₃, or domain 4) is indicated for each clone.

Reason, D., Liberato, J., Sun, J., Keitel, W., & Zhou, J. (2009). Frequency and domain specificity of toxin-neutralizing paratopes in the human antibody response to anthrax vaccine adsorbed. Infection and immunity, 77(5), 2030-2035.

Toxin-neutralizing antibodies were preincubated with a receptor-deficient mutant of PA (PArb−) or the amino-terminal 191 residues of PA expressed as a GFP fusion protein (PA₂₀-GFP) prior to their addition to the RAW267.4 cytotoxicity assay mixture. Open bars represent the degree of inhibition observed when antibodies were preincubated with PArb−. Filled bars represent inhibition by PA₂₀-GFP. Antibodies are grouped by their domain specificity.

Reason, D., Liberato, J., Sun, J., Keitel, W., & Zhou, J. (2009). Frequency and domain specificity of toxin-neutralizing paratopes in the human antibody response to anthrax vaccine adsorbed. Infection and immunity, 77(5), 2030-2035.

CHO-derived human antibodies specific for PA were titrated in the RAW 264.7 cytotoxicity assay and the amount required to bring about 50% cell death determined. The median value for each group is indicated by a horizontal line. PA₆₃- and PA₂₀-specific antibodies differed significantly in the concentration required to effect 50% neutralization of LT (P = 0.01 by the Mann-Whitney U test).

Reason, D., Liberato, J., Sun, J., Keitel, W., & Zhou, J. (2009). Frequency and domain specificity of toxin-neutralizing paratopes in the human antibody response to anthrax vaccine adsorbed. Infection and immunity, 77(5), 2030-2035.