Recombinant Mouse Antibody (mAb8) is capable of binding to CPV, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Binding of the scFv in ELISA to CPV (A) or FPV (B) capsids or to CPV denatured by boiling (C).
The reactions are compared with the binding of the original MAb of the same antibodies, and in (C) a rabbit serum (Anti-CPV), which recognizes both assembled and denatured capsid proteins, was also included.
Yuan, W., & Parrish, C. R. (2000). Comparison of two single-chain antibodies that neutralize canine parvovirus: analysis of an antibody-combining site and mechanisms of neutralization. Virology, 269(2), 471-480.
Figure 2 Flow cytometric analysis of the binding of CPV capsids andcanine Tf to TRVb cells transiently expressing the feline TfR or receptors with altered transmembrane or cytoplasmic sequences.
Cells were incubated with Cy5-labeled Tf and CPV together for 1h at 37°C,and then after detachment from the tissue culture dishes and fixation,the cell-associated CPV was detected with Cy2-labeled MAb 8. y axis,Tf binding; x axis, capsid binding.
Hueffer, K., Palermo, L. M., & Parrish, C. R. (2004). Parvovirus infection of cells by using variants of the feline transferrin receptor altering clathrin-mediated endocytosis, membrane domain localization, and capsid-binding domains. Journal of virology, 78(11), 5601-5611.
Figure 3 Neutralization by scFv compared with that by intact IgG of the same Mab.
Titrations were performed in NLFK cells, and the TCID₅₀ remaining after incubation in the presence of dilutions of the antibody are shown.
Yuan, W., & Parrish, C. R. (2000). Comparison of two single-chain antibodies that neutralize canine parvovirus: analysis of an antibody-combining site and mechanisms of neutralization. Virology, 269(2), 471-480.
Figure 4 CPV infection of cells microinjected simultaneously with MAb 8 (5mg/ml) and the infectious plasmid clone of CPV (200 µg/ml).
(A) MAb 8detected using FITC-labeled goat anti-mouse IgG (green) and viral infectionvisualized with TxR-labeled anti-NS1 antibody (red). The insert shows anothercell subjected to the same treatment. (B) Capsids were present within the ag-gregates seen in the cells. Injected MAb 8 was detected using FITC-labeled goatanti-mouse IgG (green), while capsids were detected using rabbit polyclonalanti-capsid IgG and TxR-conjugated anti-rabbit antibody (red). The insert showsanother cell subjected to the same treatment.
Vihinen-Ranta, M., Yuan, W., & Parrish, C. R. (2000). Cytoplasmic trafficking of the canine parvovirus capsid and its role in infection and nuclear transport. Journal of virology, 74(10), 4853-4859.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-237-S(P) | Recombinant Anti-CPV Antibody scFv Fragment | Neut, IF, FuncS | scFv |
MHH-237-S(P) | Recombinant Human Anti-CPV Antibody scFv Fragment | FC, IP, FuncS | scFv |
PFBW-140 | Recombinant Mouse Anti-CPV Antibody Fab Fragment (mAb16) | Neut | Fab |
PFBW-141 | Recombinant Mouse Anti-CPV Antibody Fab Fragment (mAb15) | Neut | Fab |
PFBW-142 | Recombinant Mouse Anti-CPV Antibody Fab Fragment (mAb8) | Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-237-F(E) | Recombinant Human Anti-CPV Antibody Fab Fragment | WB, Neut, FuncS | Fab |
PFBL-432 | Recombinant Mouse Anti-CPV Antibody Fab Fragment (730.1.4) | WB, IF, FuncS | Fab |
PSBW-140 | Recombinant Mouse Anti-CPV Antibody scFv Fragment (mAb16) | Neut | scFv |
PSBW-145 | Recombinant Mouse Anti-CPV Antibody scFv Fragment (mAb14) | Neut | scFv |
PFBC-482 | Recombinant Rat Anti-CPV Antibody Fab Fragment (MAb E) | ELISA, Neut | Rat Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-435 | Recombinant Mouse Anti-CPV Antibody (730.1.4) | WB, IF, FuncS | IgG |
PABW-140 | Recombinant Mouse Anti-CPV Antibody (mAb16) | Neut, ELISA | IgG |
PABW-141 | Recombinant Mouse Anti-CPV Antibody (mAb15) | Neut, ELISA | IgG |
PABW-143 | Recombinant Mouse Anti-CPV Antibody (mAb6) | Neut | IgG |
HPAB-A1274-YJ | Cat Anti-CPV Recombinant Antibody (clone CPV) | WB, ELISA, Neut, Inhib | Cat IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-023 | Recombinant Mouse Anti-CPV Antibody (FabB) | WB, ELISA, FuncS | IgG |
PABX-023-S (P) | Recombinant Mouse Anti-CPV Antibody scFv Fragment (FabB) | WB, ELISA, FuncS | scFv |
PABX-023-F (E) | Recombinant Mouse Anti-CPV Antibody Fab Fragment (FabB) | WB, ELISA, FuncS | Fab |
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