Mouse Anti-Cocaine benzoyl ester Recombinant Antibody (clone 15A10) (CAT#: PABZ-028)
Recombinant Mouse Antibody (15A10) is capable of binding to Cocaine benzoyl ester, expressed in Chinese Hamster Ovary cells (CHO). Catalytic antibody 15A10 hydrolyzes the benzoyl ester of cocaine to form the nonpsychoactive metabolites benzoic acid and ecgonine methylester. 15A10 hydrolyzes the benzoyl ester of cocaine to yield the nonpsychoactive metabolite ecgonine methylester with a kcat of 2. 3 min-1, KM of 220 μM, and kcatkuncat-1 =2. 3x104.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 Log dose-response relationship for mAb 15A10 on rats' survival after infusion of an LD90 (16 mg/kg) cocaine.
The effect of mAb 15A10 on survival was significant by a X2 test (P < 0.001).
Mets, B., Winger, G., Cabrera, C., Seo, S., Jamdar, S., Yang, G., ... & Landry, D. W. (1998). A catalytic antibody against cocaine prevents cocaine’s reinforcing and toxic effects in rats. Proceedings of the National Academy of Sciences, 95(17), 10176-10181.
Specifications
- Immunogen
- Cocaine
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- 15A10
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 15A10 has been reported in applications of Functional Assay, Blocking, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Functional Assay: Rats pretreated with mAb 15A10 showed a significant dose-dependent increase in survival after an LD90 cocaine infusion. The concentration of 15A10 was 5 mg/kg-50 mg/kg.
Blocking: It is reported that the administration of Mab 15A10 (9 mg) completely blocked the reinforcing effect of cocaine in rat model.
Target
- Alternative Names
- Methyl (1R; 2R; 3S; 5S)-3-(benzoyloxy)-8-methyl-8 azabicyclo[3.2.1] octane-2-carboxylate; Cocaine; benzoylmethylecgonine; Bernice; Bernies
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 15A10"
See other products for "Cocaine benzoyl ester"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-028 | Mouse Anti-Cocaine benzoyl ester Recombinant Antibody (clone 15A10); Fab Fragment | ELISA | Mouse Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABZ-028. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, ELISA, FuncS
Application: WB, ELISA, FuncS
-CB2006C17L-4.jpg)
Application: WB, ELISA
-2-1.png)
Application: ELISA
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA, Inhib, FuncS
Application: ELISA, WB, Microarray, Block
Application: Inhib, FC, WB
Application: ELISA, FuncS
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
