Hi-Affi™ Rabbit Anti-DDX21 Recombinant Antibody (clone DS957AB) (CAT#: MOR-0957)
Recombinant Rabbit anti-DDX21 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/430_D9_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/430_D9_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36593/592_E5_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36593/592_E5_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/1888_B12_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/1888_B12_2_red_green.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_3.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_5.jpg
(Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_5.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_B_7_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36592/74208_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165732-DDX21
Specifications
- Immunogen
- Synthetic peptide within Human DDX21 aa 650-750.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human DDX21
- Species Reactivity
- Human
- Clone
- DS957AB
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- DEAD (Asp-Glu-Ala-Asp) Box Helicase 21; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD Box Protein 21; RH II/Gu; Gu-Alpha; RNA Helicase II/Gu Alpha; EC 3.6.4.13; Gu Protein; RH-II/GuA; RH-II/GU; EC 3.6.1; GURDB; GUA
- Gene ID
- 9188
- UniProt ID
- Q9NR30
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1325z | Mouse Anti-DDX21 Recombinant Antibody (clone 16A4) | WB, IHC, IF, ELISA | Mouse IgG1 |
| VS3-FY448 | Recombinant Rabbit Anti-DDX21 Antibody (clone R04-4J2) | WB, ICC, IF | Rabbit IgG |
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