Recombinant Anti-E. coli Etk Intrabody [Tat] (CAT#: IAB-B062(T))
Recombinant anti-E. coli Etk intrabody in fusion with HIV Tat works within cells to bind E. coli Etk, expressed in E. coli cells. The intrabody is specific for the kinase domain of Etk and reduces or inhibits autophosphorylation or phosphorylation of a substrate.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Immunogen
- Escherichia coli tyrosine-protein kinase, role in O-antigen capsule formation
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- E. coli
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
Target
- Alternative Names
- E. coli Etk; Escherichia coli tyrosine-protein kinase, role in O-antigen capsule formation
- Gene ID
- 947409
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Intrabody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B062(A) | Recombinant Anti-E. coli Etk Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B062(G) | Recombinant Anti-E. coli Etk Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
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