Anti-ELAVL4 immunotoxin BW-2 (IgG)-Sap (CAT#: AGTO-G020S)
This immunotoxin was achieved by conjugating/fusing the Anti-ELAVL4 monoclonal antibody to saporin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells and enters cells by receptor-mediated endocytosis. Upon release of the conjugate from endosomes, the saporin toxin catalytically renders the large subunit ribosomes unable to bind the elongation factors and inhibits protein synthesis. It was designed for treatment of lung cancer and neuroblastoma.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1252_H2_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1252_H2_4_selected.jpg
Figure 2 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1734_E3_2_red_green.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1734_E3_2_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1235_E1_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43047/1235_E1_2_red_green.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_B_8_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_B_8_5.jpg
Figure 5 Colon
(Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_A_7_3.jpg
(Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_A_7_3.jpg
Figure 6 Testis
(Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_A_6_6.jpg
(Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4442/168341_A_6_6.jpg
Figure 7 RNA cell line category: Cell line enriched (SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162374-ELAVL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162374-ELAVL4
Figure 7 RNA cell line category: Cell line enriched (SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162374-ELAVL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162374-ELAVL4
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- BW-2
- Molecule Class
- IgG-Sap
- Toxin Description
- Saporin is a so-called ribosome inactivating protein (RIP), due to its N-glycosidase activity, from the seeds of Saponaria officinalis (common name: soapwort).Saporin depurinate the ribosomal RNA in a specific position; rendering the large subunit of ribosomes unable to bind the elongation factors. The consequent arrest of protein synthesis is responsible for the cytotoxicity.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- lung cancer and neuroblastoma
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ELAVL4"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1322z | Mouse Anti-ELAVL4 Recombinant Antibody (clone 40C2) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| MOB-1384MZ | Recombinant Mouse Anti-Human ELAVL4 Antibody | IHC-P, WB | Mouse antibody |
| VS13-YC354 | Rabbit Anti-ELAVL4 Recombinant Antibody (VS13-YC354) | WB, IP | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for AGTO-G020S. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, Neut, FuncS
Application: ELISA, Neut
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
