Recombinant Mouse Anti-EV71 VP1 Antibody (E18) (CAT#: PABC-146)

Figure 1 Neutralization of EV71 by monoclonal antibodies E18 and E19.

Whole IgG and Fab fragments of the monoclonal antibodies E18 (A) and E19 (B) were used to inhibit EV71 at different concentrations.

Plevka, P. , Lim, P. Y. , Perera, R. , Cardosa, J. , Suksatu, A. , & Kuhn, R. J. , et al. (2014). Neutralizing antibodies can initiate genome release from human enterovirus 71. Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2134-9.

Figure 2 Analysis of binding of E18 and E19 to EV71 viral proteins by immunoblot.

(A) Mock- (M) and EV71-infected (V) cell lysates were separated by SDS/PAGE and transferred onto membrane. Membrane was probed with R525, E18, or E19. E18 and E19 did not bind denatured viral proteins and, therefore, recognize conformational epitopes.

Plevka, P. , Lim, P. Y. , Perera, R. , Cardosa, J. , Suksatu, A. , & Kuhn, R. J. , et al. (2014). Neutralizing antibodies can initiate genome release from human enterovirus 71. Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2134-9.

Figure 3 Analysis of binding of E18 and E19 to EV72 viral proteins by indirect ELISA.

(B) Indirect ELISA was performed by coating wells with recombinant viral proteins or heat-inactivated EV71-infected cell lysates.

Plevka, P. , Lim, P. Y. , Perera, R. , Cardosa, J. , Suksatu, A. , & Kuhn, R. J. , et al. (2014). Neutralizing antibodies can initiate genome release from human enterovirus 71. Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2134-9.

Figure 4 Stability of EV71 virions and their complexes with E18 and E19.

(A) Plot of time and temperature dependence of interaction of the fluorescent SYBR Green I and II dyes with the EV71 genomic RNA. Native EV71 virions (blue line) as well as EV71 complexes with E18 (green line) and E19 (red line) were incubated with the fluorescent dyes at 37 °C. The purple line represents a negative control without virus. Subsequently, the complexes were gradually heated to 90 °C. The increase in fluorescence showed that the fluorescent dyes were binding to the genomic RNA. The numbers at 60, 120, and 360 min associated with each of the lines show the percentages of empty particles in cryo-EM images for each sample. Cryo-EM images of (B) EV71 virions and of (C) EV71 complexed with E19 Fab and (D) EV71 complexed with E18 Fab that were incubated at 37 °C for 120 min. (E and F) Also shown are virions in the process of genome release observed in the E18–EV71 mixture. (Scale bars: 50 nm.)

Plevka, P. , Lim, P. Y. , Perera, R. , Cardosa, J. , Suksatu, A. , & Kuhn, R. J. , et al. (2014). Neutralizing antibodies can initiate genome release from human enterovirus 71. Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2134-9.