Anti-EpCAM Antibody-Cytokine Fusion Protein, scFv (HuKS)-IL12 (CAT#: ACFP-SH032)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM scFv to IL12. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EpCAM as well as the biological activity of IL12. This fusion protein is directed against EpCAM and enhances the uptake of immunocytokine into tumors. This immunocytokine was designed for treating solid tumor.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
Figure 2 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
Figure 4 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
Specifications
- Target
- EpCAM
- Molecule Class
- scFv-IL12
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- HuKS
- Related Disease
- Solid tumors
- Cytokine 1
- IL12
- Cytokines 1 Description
- Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component; chromosome 4; surface marker (35kD glycoprotein)
- Gene ID
- 4072
- UniProt ID
- P16422
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone huKS"
See other products for "EPCAM"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody
Immunotoxin
Mouse Antibody
Humanized Antibody
Rabbit Monoclonal Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
Recombinant Antibody
scFv Fragment Antibody
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-106 | Anti-Human EPCAM Recombinant Antibody (Adecatumumab) | FC, IHC, FuncS, Inhib, Cyt | IgG1 |
| TAB-356MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (3-171) | FC, ELISA | Human antibody |
| TAB-356MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (3-171) | FC, ELISA | Human antibody |
| TAB-357MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (7-F17) | FC, ELISA | Human antibody |
| TAB-358MZ-F(E) | Anti-Human EPCAM Recombinant Antibody Fab Fragment (12-C15) | FC, ELISA | Human antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ACFP-SH032. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: Neut, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA
-1.jpg)
Application: FC, FuncS, IA, IF, IP, IHC
Application: Inhib, ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
