Mouse Anti-FLAG Recombinant Antibody (clone M2) (CAT#: PABW-045)
Recombinant Mouse Antibody (M2) is capable of binding to FLAG, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
Figure 1 GPER1 N-glycosylation.
A, HEK293 cells stably expressing mouse FGPER1 (HEK-FGPER1) were lysed, immunoprecipitated with mouse M2 FLAG antibody-agarose, treated without (lane 1) and with PNGase F (lane 2), and immunoblotted with M2 FLAG antibody. B, MDCK cells (MDCK-FGPER1, lanes 1 and 2) and T47-D cells (T47-D-FGPER1, lanes 3 and 4) transiently transfected with FGPER1 cDNA were lysed, immunoprecipitated with M2 FLAG antibody beads, treated without (lanes 1 and 3) and with PNGase F (lanes 2 and 4), and immunoblotted with M2 FLAG antibody. Molecular mass standards (in kilodaltons) (left side arrows) are indicated. The results are representative of experiments performed at least three times.
Sandén, C., Broselid, S., Cornmark, L., Andersson, K., Daszkiewicz-Nilsson, J., Mårtensson, U. E., ... & Leeb-Lundberg, L. F. (2011). G protein-coupled estrogen receptor 1/G protein-coupled receptor 30 localizes in the plasma membrane and traffics intracellularly on cytokeratin intermediate filaments. Molecular pharmacology, 79(3), 400-410.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- M2
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The FLAG antibody has been reported in applications of Western Blot.
Target
- Alternative Names
- FLAG-tag; FLAG octapeptide; FLAG epitope
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone M2"
See other products for "flaG"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-002CQ | Rat Anti-FLAG Recombinant Antibody (clone YC011), Alexa Fluor® 488 | WB, IF, ICC | Rat IgG2a |
| MRO-0186YJ | Rabbit Anti-FLAG Polyclonal Antibody | WB, IP | Rabbit IgG |
| ZG-0350J | Mouse Anti-Flag Recombinant Antibody (ZG-0350J) | WB, ELISA | Mouse IgG |
| VS3-CJ2 | Mouse Anti-Flag Recombinant Antibody (clone G191) | WB, IP | Mouse IgG2b |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABW-045. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.