What is the original clone and isotype of this anti-CD19 antibody?

This recombinant antibody (TAB-431CQ) is based on the well-established mouse clone FMC63. It has been engineered as a humanized antibody and is expressed with a Human IgG1 isotype. This format is often used in research to provide human effector functions or to improve compatibility in human-based assay systems. It is expressed in CHO cells and has a purity of>95% by SDS-PAGE.

I need to use this antibody for immunofluorescence. Is this a validated application?

Yes, Immunofluorescence (IF) is listed as a validated application for this Anti-Human CD19 antibody. In addition to IF, it is also specified for use in ELISA, Flow Cytometry (FC), Western Blot (WB), Immunoprecipitation (IP), and Functional Studies (FuncS). This makes it a highly versatile antibody for B-cell research, targeting the human CD19 antigen.

Anti-Human CD19 Recombinant Antibody

CAT#: TAB-431CQ

Recombinant monoclonal antibody to CD19. This product is a humanized monoclonal antibody that can be potentially used in the treatment of Chronic lymphocytic leukemia (CLL), Multiple sclerosis (MS).

Tested Data Gene Expression

ELISA

Figure 1 Anti-CD19 Antibody in ELISA.

ELISA analysis of TAB-431CQ was performed by coating with Human CD19 Protein.

SDS-PAGE

Figure 2 Anti-Human CD19 Recombinant Antibody ADCC enhanced (TAB-431CQ-ADCC) in SDS-PAGE

SDS-PAGE analysis of TAB-431CQ-ADCC in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

SEC-HPLC

Figure 3 Anti-Human CD19 Recombinant Antibody ADCC enhanced (TAB-431CQ-ADCC) in SEC-HPLC

HPLC analysis of TAB-431CQ-ADCC shows that the purity is more than 95%.

ELISA

Figure 4 Anti-Human CD19 Recombinant Antibody ADCC enhanced (TAB-431CQ-ADCC) in ELISA

ELISA analysis of TAB-431CQ-ADCC was performed by coating with Recombinant Human CD19 Protein (ECD, His Tag).

The secondary antibody: HRP-Goat anti-Human IgG

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Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized

Type
IgG1, κ

Specificity
Human CD19

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies

Conjugate
Unconjugated

Related Disease
Chronic lymphocytic leukemia (CLL), Multiple sclerosis (MS)

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Target

Alternative Names
CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; CD19 Antigen; CVID3; B4

Gene ID
930

UniProt ID
P15391

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Excellent Anti-CD19 Antibody

This antibody (TAB-431CQ) is a high-quality reagent for B-cell research. It's a humanized IgG1 antibody that recognizes human CD19. The high purity (>95% by SDS-PAGE) and the fact that it's expressed in CHO cells are key quality indicators for me.

Good for FC and IF

I needed a reliable antibody for both flow cytometry (FC) and immunofluorescence (IF), and this product lists both as applications. It's derived from the mouse clone FMC63, which is a very well-known and characterized clone for CD19, giving me extra confidence in its performance.

Q&As

What is the original clone and isotype of this anti-CD19 antibody?

A: This recombinant antibody (TAB-431CQ) is based on the well-established mouse clone FMC63. It has been engineered as a humanized antibody and is expressed with a Human IgG1 isotype. This format is often used in research to provide human effector functions or to improve compatibility in human-based assay systems. It is expressed in CHO cells and has a purity of>95% by SDS-PAGE.
I need to use this antibody for immunofluorescence. Is this a validated application?

A: Yes, Immunofluorescence (IF) is listed as a validated application for this Anti-Human CD19 antibody. In addition to IF, it is also specified for use in ELISA, Flow Cytometry (FC), Western Blot (WB), Immunoprecipitation (IP), and Functional Studies (FuncS). This makes it a highly versatile antibody for B-cell research, targeting the human CD19 antigen.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-431CQ, RRID: AB_3111943)

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-431CQ.pdf

MSDS

MSDS - TAB-431CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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