What immunogen was used to generate this antibody?

This antibody was generated using NIH 3T3 cells transfected with an expression vector for IGF-IR, along with 20 μg of soluble extracellular domain of IGF-IR. This ensures the antibody recognizes the native conformation of the receptor.

Is this antibody suitable for flow cytometry?

Yes, this recombinant human monoclonal antibody has been reported for use in Flow Cytometry (FC). It is also suitable for Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), and Immunoprecipitation (IP) assays involving the IGF-1R.

Anti-Human IGF-1R Recombinant Antibody (TAB-052ZJ)

CAT#: TAB-052ZJ

Recombinant monoclonal antibody to insulin-like growth factor 1 receptor. This is a human antibody that can be potentially used in the treatment of cancer and tumor disease.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_7_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_6_6.jpg

Normal Tissue Figure 6 Heart muscle

Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_5_6.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140443-IGF1R

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Specifications

Immunogen
NIH 3T3 cells, transfected with an expression vector for IGF-IR, and 20 μg soluble extracellular domain of IGF-IR

Host Species
Human

Type
Human antibody

Specificity
Human

Species Reactivity
Human

Applications
IHC, ELISA, FC, IF, IP

Related Disease
Cancer, Tumor disease

Applications

Application Notes
This antibody has been tested for use in Immunohistochemistry, ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.

Target

Alternative Names
IGF1R; insulin-like growth factor 1 receptor; IGFR; CD221; IGFIR; JTK13; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor

Gene ID
3480

UniProt ID
P08069

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Breast cancer biomarkers at key points during disease progression

Comprehensive Application Profile

We have tested this antibody in several applications including flow cytometry and immunoprecipitation. It consistently recognizes the human IGF-1R. The fact that it is a recombinant human antibody adds to its reliability and specificity.

Excellent For IHC

We used this antibody for immunohistochemistry on tumor tissue samples. It clearly stained the insulin-like growth factor 1 receptor, helping us visualize overexpression patterns. A very robust reagent for cancer research studies.

Q&As

What immunogen was used to generate this antibody?

A: This antibody was generated using NIH 3T3 cells transfected with an expression vector for IGF-IR, along with 20 μg of soluble extracellular domain of IGF-IR. This ensures the antibody recognizes the native conformation of the receptor.
Is this antibody suitable for flow cytometry?

A: Yes, this recombinant human monoclonal antibody has been reported for use in Flow Cytometry (FC). It is also suitable for Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), and Immunoprecipitation (IP) assays involving the IGF-1R.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Signaling Pathways

Insulin Signaling Pathway

Related Diseases

Endocrine Resistance

Type I Diabetes Mellitus

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-052ZJ.pdf

MSDS

MSDS - TAB-052ZJ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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