Recombinant Anti-human NUP88 Intrabody [+36 GFP] (CAT#: IAB-B063(G))
Recombinant anti-NUP88 intrabody in fusion with +36GFP works within cells to bind the nuclear pore protein NUP88, expressed in E. coli cells. Anti-Nup88 intrabody, an anti-sense oligonucleotide or nucleic acid which is complementary to at least a functional part of a Nup88 RNA or wherein the compound is a peptide, peptide-like or non-peptide Nup88 inhibitor which can be identified by contacting the compound or libraries of peptido- mimetics, peptides, DNA or cDNA expression, combinatorial chemistry or phage display with substantially purified Nup88, fragments thereof or structural analogues thereof.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows nuclear membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2209/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows nuclear membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2209/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_4.jpg
Figure 6 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_4_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_4_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_9_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21816/49954_A_6_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108559-NUP88
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108559-NUP88
Specifications
- Immunogen
- human nucleoporin 88kDa
- Host Species
- rabbit
- Type
- scFv-(+36GFP)
- Cell Penetrating Peptide
- +36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Protein fragment complementation; Western blot; Functional studies
Product Property
- Purity
- >95% (SDS-PAGE)
- Storage
- Store at – 20 or -70°C upon receipt. Divide antibody into aliquots prior usage and avoid multiple freeze-thaw cycles.
Target
- Alternative Names
- NUP88; nucleoporin 88kDa; nuclear pore complex protein Nup88; karyoporin; nucleoporin 88kD; nucleoporin Nup88; 88 kDa nucleoporin; nuclear pore complex protein 88
- Gene ID
- 4927
- UniProt ID
- Q99567
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Intrabody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2733z | Mouse Anti-NUP88 Recombinant Antibody (clone 39C9) | WB, ELISA, IF, IP | Mouse IgG2b, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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