Hi-Affi™ Recombinant Rabbit Anti-RANBP1 Monoclonal Antibody (DS2993AB) (CAT#: MOR-2993)
Recombinant Rabbit anti-RANBP1 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65868/1494_C12_2_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65868/1494_C12_2_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong positivity in seminiferous cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_4_6_selected.jpg
(Immunohistochemical staining of human testis shows strong positivity in seminiferous cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_4_6_selected.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162116_B_6_1.jpg
(Squamous epithelial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162116_B_6_1.jpg
Figure 4 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_7_3.jpg
Figure 5 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65931/162102_A_7_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099901-RANBP1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099901-RANBP1
Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2993AB
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3448z | Mouse Anti-RANBP1 Recombinant Antibody (clone 39G12) | WB, IP, IF, ELISA | Mouse IgG1, κ |
Customer Reviews and Q&As
Customer Review
Q&As
Reliable for Western Blotting
As someone who frequently conducts Western Blots, I found the Hi-Affi™ Recombinant Rabbit Anti-RANBP1 Monoclonal Antibody to be exceedingly reliable. The antibody consistently provided clear results across multiple blotting experiments, significantly aiding our studies on RANBP1 expression in cancer cells.
Enhances Research on Cancer Markers
By targeting RANBP1, associated with several cancer markers, this antibody has bolstered our research into potential cancer biomarkers. This reliability helps in experimental setups, making the process both efficient and academically fruitful.
High Specificity and Sensitivity
This antibody distinguished itself with high specificity and sensitivity, cutting down on non-specific signals and enhancing the accuracy of our Western Blot assays. It supports our protein studies by spotlighting precise RANBP1 interactions, crucial for our ongoing research on cellular dynamics.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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