Mouse Anti-VWF Recombinant Antibody (clone NMC-4) (CAT#: PABW-132)
Recombinant Mouse Antibody (NMC-4) is capable of binding to VWF, expressed in Chinese Hamster Ovary cells (CHO).
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DB
Figure 3 SDS-PAGE and dot blot analysis of the recombinant vWF fragments. A, dot blot analysis.
An aliquot of each fragment was applied onto a nitrocellulose membrane in a circular area. After drying, the membrane was treated with a solution of the anti-vWF monoclonal antibody NMC-4 (5 mg/ml) followed by fluorographic detection of the bound antibody using an alkaline phosphatase-conjugated rabbit antimouse IgG. Note that the antibody reacts well with the cyclic fragments containing the intact Cys509–Cys695 disulfide bond (C) but only minimally after reduction and alkylation of Cys residues (R/A).
Miyata, S., Goto, S., Federici, A. B., Ware, J., & Ruggeri, Z. M. (1996). Conformational changes in the A1 domain of von Willebrand factor modulating the interaction with platelet glycoprotein Ib. Journal of Biological Chemistry, 271(15), 9046-9053.
Specifications
- Immunogen
- Human Von Willebrand Factor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human VWF
- Species Reactivity
- Human
- Clone
- NMC-4
- Applications
- Block, Inhib, WB, DB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The VWF antibody has been reported in applications of Blocking, Inhibition, Western Blot, Dot Blot.
For Inhibiton: Formalin-ked platelets were prepared as previously described and used at a concentration of 10⁸ platelets/mL in an incubation volume of 125 pL. [¹²⁵I]vWF or [¹²⁵I]AS-vWF was added at a concentration of 5 or 20 µg/mL in the presence of competing ligands or MoAbs at concentrations described in Results. For vWF binding, ristocetin or botrocetin was used at a final concentration of 1 mg/mL or 15 µg/mL. Binding was measured after 30 minutes of incubation at room temperature, and nonspecific binding was determined in the presence of a 50-fold excess of unlabeledvWF or AS-vWF. Specific binding was calculated by subtracting nonspecific binding from the total.
Target
- Alternative Names
- VWF; VWD; Von Willebrand Factor; Coagulation Factor VIII VWF; VWF
- Gene ID
- 7450
- UniProt ID
- P04275
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| HPAB-0343-WJ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (HPAB-0343-WJ-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-M0250-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); Fab Fragment | Inhib, FuncS | Mouse Fab |
| HPAB-M0251-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); Fab Fragment | Inhib, FuncS | Chimeric (mouse/human) Fab |
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