Chimeric (Mouse/Human) Anti-CD20 Recombinant Antibody (TP-082CL) (CAT#: TP-082CL)
This antibody is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. The antibody is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids
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Normal Tissue
RNA Expression
Figure 1 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
Figure 2 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
Figure 3 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
Figure 4 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
Figure 5 RNA cell line category: Group enriched (Karpas-707, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
Specifications
- Type
- Biotech
Target
- Function
- Chimeric (human/mouse) mAb that binds cD20, a transmembrane protein found on over 90% of B-cell non-Hodgkin's lymphomas (NHL); synergistic effect with some small-molecule chemotherapeutic agents has been demonstrated in lymphoma cell lines
Related Resources
Chen, Haizhang, et al. "Detection and functional resolution of soluble immune complexes by an FcγR reporter cell panel." EMBO Molecular Medicine 14.1 (2022): e14182. https://doi.org/10.15252/emmm.202114182
This study focuses on developing a robust and scalable test system to detect and quantify the bioactivity of soluble IgG immune complexes (sICs). These complexes play a significant role in autoimmune diseases such as systemic lupus erythematosus (SLE). The researchers created a comprehensive panel of reporter cell lines that enable the quantification of sIC reactivity through ELISA or flow cytometry. This system identified FcγRIIA(H) and FcγRIIIA as the most sensitive receptors to sICs, allowing for the detailed analysis of sIC size and stoichiometry. The assay has shown predictive capabilities regarding the severity of SLE by analyzing sera from patients and mouse models, providing a sensitive tool to evaluate sICs in various settings.
Creative Biolabs contributed to this study by providing the pharmaceutical-grade humanized monoclonal IgG1 antibody rituximab (Cat# TP-082CL). Rituximab was crucial for generating soluble immune complexes (sICs) in the experiments. The study utilized recombinant CD20, obtained as a peptide containing the binding region of rituximab, to form these sICs. This setup allowed the researchers to create and analyze the immune complexes accurately. The high-quality rituximab provided by Creative Biolabs enabled the development of a sensitive and specific assay for detecting Fcγ receptor activation, which was essential for the study's success in evaluating the bioactivity and size of sICs.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Customer Reviews and Q&As
Customer Review
Q&As
David Walker
Excellent for Rituximab Studies
Excellent for Rituximab Studies
The Rituximab has been excellent for our CD20 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
09.Sep,23
Emily Turner
Reliable in Immunoassays
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD20 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
11.Oct,22
John Harris
Consistent and High-Quality
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for CD20 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
20.Jul,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TP-082CL, RRID: AB_3112049)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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