Mouse Anti-4-Hydroxy-2-nonenal-histidine adducts Recombinant Antibody (clone S412) (CAT#: FAMAB-0136JF)
Recombinant mouse anti-S monoclonal antibody S412 specially binds to 4-Hydroxy-2-nonenal-histidine adducts. This product can be useful in further investigating the anti-HNE immune response. This antibody was originally generated in mice immunized with the R-HNE modified and S-HNE-modified keyhole limpet hemocyanins.
Specific Inquiry
WB
Figure 1 Western blot analysis of mAbs HNEJ-15 (200 ug/ml) using BSA and HNE-BSA.
1-5 correspond to mAbs HNEJ-1 5, respectively.
Toyokuni, S., Miyake, N., Hiai, H., Hagiwara, M., Kawakishi, S., Osawa, T., & Uchida, K. (1995). The monoclonal antibody specific for the 4‐hydroxy‐2‐nonenal histidine adduct. FEBS letters, 359(2-3), 189-191.
ELISA
Figure 2 ELISA competition curves for mAb HNEJ-2.
Assays used HNE-modified GAPDH as the absorbed antigen.
Toyokuni, S., Miyake, N., Hiai, H., Hagiwara, M., Kawakishi, S., Osawa, T., & Uchida, K. (1995). The monoclonal antibody specific for the 4‐hydroxy‐2‐nonenal histidine adduct. FEBS letters, 359(2-3), 189-191.
IHC
Figure 3 Immunohistochemistry of renal cortex with mAbS412.
The immunoreactivities with mAbS412 were mainly detected in the cytoplasm and in some of the nuclei, whereas the immunoreactivities with mAbR310
were mainly detected in the nuclei.
Hashimoto, M., Sibata, T., Wasada, H., Toyokuni, S., & Uchida, K. (2003). Structural Basis of Protein-bound Endogenous Aldehydes CHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATIONS OF CONFIGURATIONAL ISOMERS OF A 4-HYDROXY-2-NONENAL-HISTIDINE ADDUCT. Journal of Biological Chemistry, 278(7), 5044-5051.
FuncS
Figure 4 Binding of mAbS412 to the configurational isomers of HNE-histidine adducts.
The HNE-modified BSA was used as the absorbed antigens.
Hashimoto, M., Sibata, T., Wasada, H., Toyokuni, S., & Uchida, K. (2003). Structural Basis of Protein-bound Endogenous Aldehydes CHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATIONS OF CONFIGURATIONAL ISOMERS OF A 4-HYDROXY-2-NONENAL-HISTIDINE ADDUCT. Journal of Biological Chemistry, 278(7), 5044-5051.
Specifications
- Immunogen
- R-HNE modified and S-HNE-modified keyhole limpet hemocyanins
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- 4-Hydroxy-2-nonenal-histidine adducts
- Clone
- S412
- Applications
- WB, ELISA, IHC, FuncS
- Related Disease
- SLE
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The 4-Hydroxy-2-nonenal-histidine adducts antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Functional Assay.
Target
- Alternative Names
- 4-Hydroxy-2-nonenal-histidine adducts; S-HNE-histidine adducts
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone S412"
- FAMAB-0136JF-F(E)
- Mouse Anti-4-Hydroxy-2-nonenal-histidine adducts Recombinant Antibody (clone S412); Fab Fragment
See other products for "4-Hydroxy-2-nonenal-histidine adducts"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0136JF-F(E) | Mouse Anti-4-Hydroxy-2-nonenal-histidine adducts Recombinant Antibody (clone S412); Fab Fragment | ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0136JF-S(P) | Mouse Anti-4-Hydroxy-2-nonenal-histidine adducts Recombinant Antibody (clone S412); scFv Fragment | ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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