Recombinant Mouse Anti-A. castellanii Antibody (MTAC4) (CAT#: FAMAB-0011YC)
Acanthamoeba species are ubiquitous soil and freshwater protozoa that have been associated with infections of the human brain, skin, lungs and eyes. The specific antibody, clone MTAC4, binds to cysts and trophozoites of the environmental group I species, Acanthamoeba
tubiashi.
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FC
Figure 1 Mouse Anti-A. castellanii Antibody (MTAC4) in FC.
Flow cytometry histograms demonstrating reactivity of eight monoclonal antibodies with cysts and trophozoites of unfixed Acanthamoeba castellanii AC112.
Turner, M. L. , Cockerell, E. J. , Brereton, H. M. , Badenoch, P. R. , Tea, M. , & Coster, D. J. , et al. (2005). Antigens of selected acanthamoeba species detected with monoclonal antibodies. International Journal for Parasitology, 35(9), 981-990.
IHC
Figure 2 Mouse Anti-A. castellanii Antibody (MTAC4) in IHC.
Indirect immunoperoxidase staining on methanol-fixed Acanthamoeba castellanii AC112 (A, B) cysts and (C, D) trophozoites, with MTAC4 (B, D) and LION1, a negative control monoclonal antibody of IgM isotype (A, C). No counterstain was applied to the preparations. Original magnification×320. Scale bar = 10 μm.
Turner, M. L. , Cockerell, E. J. , Brereton, H. M. , Badenoch, P. R. , Tea, M. , & Coster, D. J. , et al. (2005). Antigens of selected acanthamoeba species detected with monoclonal antibodies. International Journal for Parasitology, 35(9), 981-990.
IHC
Figure 3 Mouse Anti-A. castellanii Antibody (MTAC4) in IHC.
Indirect immunoperoxidase staining with MTAC3, MTAC4 and AMEC1 on sections of an Acanthamoeba-infected human cornea. No counterstain was applied to the preparations. The anterior surface of the cornea is on the left. (A) MTAC3; (B) MTAC4; (C) MTAC4; (D) AMEC1; (E) MTAC3; (F) Giemsa-stained section of the same cornea shown for comparative purposes. Original magnification: ×120 (panels A, B, F), ×320 (panels C, D, E). Scale bar = 10 μm.
Turner, M. L. , Cockerell, E. J. , Brereton, H. M. , Badenoch, P. R. , Tea, M. , & Coster, D. J. , et al. (2005). Antigens of selected acanthamoeba species detected with monoclonal antibodies. International Journal for Parasitology, 35(9), 981-990.
Specifications
- Immunogen
- Heat-treated trophozoites
- Host Species
- Mouse
- Type
- Mouse IgM, κ
- Specificity
- A. castellanii
- Species Reactivity
- A. castellanii
- Clone
- MTAC4
- Applications
- Flow Cytometry, Immunohistochemistry
- Related Disease
- Acanthamoeba infection
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- Acanthamoeba castellanii; A. castellanii
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0007YC | Recombinant Mouse Anti-A. castellanii Antibody (AMEC1) | FC, IHC | Mouse IgM, κ |
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