Rabbit Anti-BRCC3 Recombinant Antibody (clone R01-7E5) (CAT#: VS3-FY1669)
Clone R01-7E5 is a rabbit IgG recombinant monoclonal antibody that is designed to specifically target human BRCC3 protein, a calculated weight of 36 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-7E5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48737/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48737/if_selected.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185515-BRCC3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185515-BRCC3
Specifications
- Immunogen
- Synthetic peptide of human BRCC36
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BRCC3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7E5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Moyamoya Disease 4 With Short Stature, Hypergonadotropic Hypogonadism, And Facial Dysmorphism and Hereditary Breast Ovarian Cancer Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- BRCA1/BRCA2-Containing Complex Subunit 3; BRCA1/BRCA2-Containing Complex Subunit 36; BRCA1-A Complex Subunit BRCC36; BRISC Complex Subunit BRCC36; CXorf53; BRCC36; C6.1A
- Gene ID
- 79184
- UniProt ID
- P46736
- Sequence Similarities
- Belongs to the peptidase M67A family. BRCC36 subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Acetylation
Phosphoprotein
- Protein Refseq
- XP_005274808.1; XP_054183838.1; NP_001018065.1; NP_001229569.1; NP_077308.1
- Function
- Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.
Does not have activity toward 'Lys-48'-linked polyubiquitin chains.
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRCC3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1483MZ | Mouse Anti-BRCC3 Recombinant Antibody (clone BU4C2) | ELISA, IHC-P, WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0381 | Hi-Affi™ Rabbit Anti-BRCC3 Recombinant Antibody (clone DS381AB) | WB, ICC, IP | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4432 | PE-A*69:01/Human BRCC3 (DAYRRIHSL) MHC Tetramer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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