Recombinant Mouse Anti-C. neoformans GXM Antibody (3E5) (CAT#: FAMAB-0042CQ)
This product is a recombinant mouse antibody clone 3E5, which specifically binds to C. neoformans GXM.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 4 ELISA
A, binding of changing concentrations of mAbs 3E5 IgA and 3E5 IgE to immobilized GXM. Isotype-specific secondary Abs were used as well as anti-κ secondary Ab. The inset represents the schematic for the ELISA configuration. B, competition ELISA of mAb 3E5 IgE with changing concentrations of mAb 3E5 IgA to immobilized GXM. Secondary Ab used is goat anti-mouse-alkaline phosphatase (GAM-AP) IgE. The schematic represents the ELISA configuration.
Janda, A., Eryilmaz, E., Nakouzi, A., Pohl, M. A., Bowen, A., & Casadevall, A. (2015). Variable region identical IgA and IgE to Cryptococcus neoformans capsular polysaccharide manifest specificity differences. Journal of Biological Chemistry, 290(19), 12090-12100.
IF
Figure 5 Immunofluorescence of strain 24067 stained with mAbs 3E5 IgA and 3E5 IgE.
Janda, A., Eryilmaz, E., Nakouzi, A., Pohl, M. A., Bowen, A., & Casadevall, A. (2015). Variable region identical IgA and IgE to Cryptococcus neoformans capsular polysaccharide manifest specificity differences. Journal of Biological Chemistry, 290(19), 12090-12100.
FuncS
Figure 6 A, J774 cells and C. neoformans were used to study the phagocytosis efficacy of the mAbs. B, survival of Balb/c mice with either NSO or the two variants of 3E5 mAb. The Abs were administered 2 h before infection.
Janda, A., Eryilmaz, E., Nakouzi, A., Pohl, M. A., Bowen, A., & Casadevall, A. (2015). Variable region identical IgA and IgE to Cryptococcus neoformans capsular polysaccharide manifest specificity differences. Journal of Biological Chemistry, 290(19), 12090-12100.
Specifications
- Immunogen
- C. neoformans cells
- Host Species
- Mouse
- Type
- Mouse IgA
- Specificity
- Cryptococcus neoformans capsular polysaccharides glucuronoxylomanan
- Species Reactivity
- C. neoformans
- Clone
- 3E5
- Applications
- ELISA, IF, IHC, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry and Functional Assay.
Target
- Alternative Names
- C. neoformans GXM; Cryptococcus neoformans capsular polysaccharides glucuronoxylomanan
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
ScFv Antibody Products
IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0042CQ-F(E) | Recombinant Mouse Anti-C. neoformans GXM Antibody Fab Fragment (3E5) | ELISA, IF | Mouse Fab |
| FAMAB-0107JF-F(E) | Mouse Anti-C. neoformans GXM Recombinant Antibody (clone G15); Fab Fragment | ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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